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DNA analysis

 
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docsubhi



Joined: 03 Sep 2007
Posts: 59
Location: london

PostPosted: Sat Aug 16, 2008 8:56 am    Post subject: DNA analysis Reply with quote

Regarding DNA analysis, it ...

a. can be used to determine fetal sex.

b. can be used to determine fetal Rhesus group.

c. can be used to determine fetal karyotype

d. can be used to determine fetal risk of spina bifida

e. has been used to diagnose Huntington disease

f. has been used to diagnose myotonic dystrophy

g. has been used to diagnose fetal HIV infection



TTFFTTF
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Maud



Joined: 11 Oct 2007
Posts: 73
Location: Bristol

PostPosted: Mon Aug 18, 2008 5:21 pm    Post subject: Reply with quote

a F, that's chromosomal analysis, isn't it? How does that determine fetal sex?
b T, can, I think, but is that how you do it?
c F, as a
d F, risk multi-factorial, including folate supplementation, drugs etc
e T, autosomal dominant
f T, autosomal recessive
g F, not any infections diagnosed this way

Can you please explain a?
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rpwalavalkar
Teale Fenning Administrator


Joined: 20 Jul 2006
Posts: 966

PostPosted: Wed Aug 20, 2008 7:20 am    Post subject: Re: DNA analysis Reply with quote

docsubhi wrote:
Regarding DNA analysis, it ...

g. has been used to diagnose fetal HIV infection [/b]


False as, there is no DNA in HIV virus to do DNA testing.
Laughing Wink


The point of discussion is --

the RT-PCR test for HIV, converts HIV RNA to cDNA and then PCR done, accuracy is good.

so is this a DNA test or a RNA test?
The question asks for a DNA test.

r
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Dr Miss. Raj Walavalkar MBBS MRCOG
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SR O&G Wessex Region
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cpeedahsa
Century Club


Joined: 21 Apr 2007
Posts: 921

PostPosted: Fri Aug 29, 2008 2:51 am    Post subject: Re: DNA analysis Reply with quote

docsubhi wrote:
Regarding DNA analysis, it ...

a. can be used to determine fetal sex.


[b]True


Yes, DNA analaysis can be used to determine fetal sex.

Actually DNA analysis allows for more rapid sex determination by assaying for Y specific sequences or by quantitative assay of X specific sequences.
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cpeedahsa
Century Club


Joined: 21 Apr 2007
Posts: 921

PostPosted: Fri Aug 29, 2008 2:55 am    Post subject: Re: DNA analysis Reply with quote

docsubhi wrote:
Regarding DNA analysis, it ...

b. can be used to determine fetal Rhesus group.

[/b]

TTFFTTF


True

Utilizing cell-free fetal DNA present in the maternal blood: various potential applications including prenatal genetic testing and screening;
the test is being considered as an alternative to standard procedures for pregnant women with Rhesus-negative blood.
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cpeedahsa
Century Club


Joined: 21 Apr 2007
Posts: 921

PostPosted: Fri Aug 29, 2008 2:58 am    Post subject: Re: DNA analysis Reply with quote

docsubhi wrote:
Regarding DNA analysis, it ...
c. can be used to determine fetal karyotype


false
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cpeedahsa
Century Club


Joined: 21 Apr 2007
Posts: 921

PostPosted: Fri Aug 29, 2008 3:01 am    Post subject: Re: DNA analysis Reply with quote

docsubhi wrote:
Regarding DNA analysis, it ...


d. can be used to determine fetal risk of spina bifida -False
e. has been used to diagnose Huntington disease -True
f. has been used to diagnose myotonic dystrophy -True
g. has been used to diagnose fetal HIV infection False
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mrcog2010



Joined: 08 Jul 2008
Posts: 32

PostPosted: Fri Sep 05, 2008 12:30 pm    Post subject: Reply with quote

Regarding DNA analysis, it ...

a. can be used to determine fetal sex. F

b. can be used to determine fetal Rhesus group. T??

c. can be used to determine fetal karyotype T

d. can be used to determine fetal risk of spina bifida T?

e. has been used to diagnose Huntington disease F

f. has been used to diagnose myotonic dystrophy F

g. has been used to diagnose fetal HIV infection F

CAN WE DISCUSS PLZ? Very Happy
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mrcog2010



Joined: 08 Jul 2008
Posts: 32

PostPosted: Fri Sep 05, 2008 12:33 pm    Post subject: Re: DNA analysis Reply with quote

cpeedahsa wrote:
docsubhi wrote:
Regarding DNA analysis, it ...

b. can be used to determine fetal Rhesus group.

[/b]

TTFFTTF


True

Utilizing cell-free fetal DNA present in the maternal blood: various potential applications including prenatal genetic testing and screening;
the test is being considered as an alternative to standard procedures for pregnant women with Rhesus-negative blood.
Any referance cpeedahsa??
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docsubhi



Joined: 03 Sep 2007
Posts: 59
Location: london

PostPosted: Sun Sep 07, 2008 10:50 pm    Post subject: Reply with quote

Hi MRCOG2010

There is a recent review in 2007 TOG
Also in Dewhurst
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mrcog2010



Joined: 08 Jul 2008
Posts: 32

PostPosted: Mon Sep 08, 2008 8:14 pm    Post subject: Reply with quote

thank you Smile
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